mi-faser is a metagenome (metagenome/metatranscriptome)
mi-faser combines faser (functional annotation of sequencing reads), an algorithm that maps reads to molecular functions encoded by the read-correspondent genes, with a manually curated reference database of protein functions. As our method is optimized for short reads, no pre-assembly is required -- just submit your raw (but quality controlled) raw data AS IS.
As output, mi-faser produces high precision sets of molecular functions identified in the microbiome sequence data. mi-faser's minutes-per-
Note that although mi-faser is specifically targeted to microbiome analysis, it could also potentially be used for the analysis of unassembled bacterial genome data as well.
Repository for source code and stand-alone version (linux/osx): https://bitbucket.org/bromberglab/mifaser_base
mifaser runs on LINUX, MacOSX and WINDOWS systems.
Open a terminal and checkout the mi-faser repository:
git clone https://email@example.com/bromberglab/mifaser_base.git
or download the zipped version:
curl --remote-name https://bitbucket.org/bromberglab/mifaser_base/get/master.zip unzip master.zip
Support online material for mi-faser manuscript: